Alcohol Dehydrogenase (ADH) anticorps (Biotin)

N° du produit ABIN1607773

Détail du produit

Antigène: Alcohol Dehydrogenase (ADH)

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.

Pureté: Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Alcohol Dehydrogenase has been assayed against 1.0 µg of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détail du antigène

Antigène: Alcohol Dehydrogenase (ADH)

Autre désignation: Alcohol Dehydrogenase (ADH Produits)

Synonymes: anticorps ADH, anticorps ALCOHOL DEHYDROGENASE, anticorps ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, anticorps ATADH, anticorps ATADH1, anticorps F22K20.19, anticorps F22K20_19, anticorps alcohol dehydrogenase 1, anticorps 2610201A18Rik, anticorps Akr1a4, anticorps ALR1, anticorps Adh, anticorps Adh1a, anticorps Adh1c, anticorps aldo-keto reductase family 1 member A1, anticorps alcohol dehydrogenase 1, anticorps aldo-keto reductase family 1, member A1 (aldehyde reductase), anticorps alcohol dehydrogenase 1 (class I), anticorps AKR1A1, anticorps ADH1, anticorps Akr1a1, anticorps Adh1

Sujet: Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1 Alcohol dehydrogenase I YADH-1

ID gène: 2538902, 12643994

UniProt: P00330