Ascorbate Oxidase anticorps (Biotin)

N° du produit ABIN1607781

Détail du produit anti-Ascorbate Oxidase anticorps (Biotin)

Antigène: Ascorbate Oxidase (AO)

Reactivité: Pumpkin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ascorbate Oxidase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.

Pureté: Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Ascorbate Oxidase has been assayed against 1.0 µg of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2.000 to 1:10.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Ascorbate Oxidase

Antigène: Ascorbate Oxidase (AO)

Autre désignation: Ascorbate Oxidase (AO Produits)

Synonymes: anticorps AO, anticorps ascorbate oxidase, anticorps L-ascorbate oxidase, anticorps AO, anticorps LOC9310099

Sujet: Anti-Ascorbate Oxidase antibody is cytoplasmic and performs binding with copper ions. Anti-Ascorbate Oxidase belongs to the multicopper oxidase family and may be a factor in the redox system involving ascorbic acid, acting as a catalyst. Found prevalently in plants, Ascorbate Oxidase has been suggested to increase plant growth. Anti-Ascorbate Oxidase antibody is ideal for researchers interested in the investigation of Cell Biology. Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3

UniProt: P24792