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Penicillinase anticorps (Biotin)

Reactivité: Enterobacter cloacae WB, IP, ELISA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN1607809
  • Antigène Tous les produits Penicillinase
    Penicillinase
    Reactivité
    • 4
    • 1
    Enterobacter cloacae
    Hôte
    • 4
    • 1
    Lapin
    Clonalité
    • 4
    • 1
    Polyclonal
    Conjugué
    • 4
    • 1
    Cet anticorp Penicillinase est conjugé à/à la Biotin
    Application
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Réactivité croisée (Details)
    Cross reactivity against Penicillinase from other sources is unknown.
    Pureté
    Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    niveau d'endotoxine
    Low Endotoxin : No
    Immunogène
    Penicillinase [Enterobacter cloacae]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Indications d'application
    Anti-Penicillinase antibody has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Conseil sur la manipulation
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Date de péremption
    Expiration date is one (1) year from date of opening.
  • Antigène
    Penicillinase
    Abstract
    Penicillinase Produits
    Synonymes
    anticorps beta-lactamase TEM-1, anticorps blaTEM-1
    Sujet
    Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria. Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody
    ID gène
    9125732
    UniProt
    P05364
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