XDH anticorps (Biotin)

N° du produit ABIN1607817

Détail du produit anti-XDH anticorps (Biotin)

Antigène: XDH (Xanthine Dehydrogenase (XDH))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XDH est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Xanthine Oxidase from other sources is unknown.

Pureté: Anti-Xanthine Oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Xanthine Oxidase [Bovine Buttermilk]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Xanthine Oxidase antibody has been assayed against 1.0 µg of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:40.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur XDH

Antigène: XDH (Xanthine Dehydrogenase (XDH))

Autre désignation: Xanthine Oxidase (XDH Produits)

Synonymes: anticorps XO, anticorps XOR, anticorps Xor, anticorps Xox-1, anticorps Xox1, anticorps XDH, anticorps im:7146167, anticorps DDBDRAFT_0219626, anticorps DDBDRAFT_0230176, anticorps DDB_0219626, anticorps DDB_0230176, anticorps xanthine dehydrogenase, anticorps xanthine dehydrogenase/oxidase, anticorps Xanthine dehydrogenase, anticorps XDH, anticorps Xdh, anticorps xdh, anticorps LOC576712, anticorps Trebr_1754

Sujet: Anti-Xanthine Oxidase recognizes xanthine oxidase, an oxidoreductase. Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid. Both of these reactions also generate the reactive oxygen species hydrogen peroxide. In addition, xanthine oxidase is involved in the catabolism of purines and metabolism of caffeine. Clinically, xanthine oxidase has been linked to gout, a condition in which uric acid accumulates in the kidneys and joints. Anti-Xanthine Oxidase antibody is ideal for investigators interested in Metabolism and Signal Transduction research. Synonyms: Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

ID gène: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity