CX3CL1 anticorps (Biotin)

N° du produit ABIN1607866

Détail du produit anti-CX3CL1 anticorps (Biotin)

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CX3CL1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity with Fractalkine from other sources has not been determined.

Pureté: Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100 % homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application: Fractalkine purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Fluorochrome Protein Value: 10-20
Western Blot Dilution: 1:1000-1:2000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with 100 µL deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur CX3CL1

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Autre désignation: Fractalkine (CX3CL1 Produits)

Synonymes: anticorps ABCD3, anticorps CX3CL1, anticorps DKFZp459K117, anticorps Cx3c, anticorps Scyd1, anticorps ABCD-3, anticorps C3Xkine, anticorps CXC3, anticorps CXC3C, anticorps NTN, anticorps NTT, anticorps SCYD1, anticorps fractalkine, anticorps neurotactin, anticorps AB030188, anticorps AI848747, anticorps CX3C, anticorps Cxc3, anticorps D8Bwg0439e, anticorps ATP binding cassette subfamily D member 3, anticorps C-X3-C motif chemokine ligand 1, anticorps chemokine (C-X3-C motif) ligand 1, anticorps ABCD3, anticorps CX3CL1, anticorps Cx3cl1, anticorps Abcd3

Sujet: Fractalkine (CX3CL1) is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. It is found predominantly in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium by binding to CX3CR1. Recombinant human Fractalkine is a non-glycosylated protein with a molecular weight of 8.6 kDa. Anti-Fractalkine antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: CX3CL1, Fractalkine, Chemokine (C-X3-C motif) ligand 1, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1, A-152E5.2

ID gène: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane