RFP anticorps (Biotin)

N° du produit ABIN1607901

Détail du produit anti-RFP anticorps (Biotin)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Fonction: Anti-RFP is designed to detect recombinant RFP.

Purification: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by ELISA, western blot and immunoelectrophoresis.

Immunogène: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1.000 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with 100 µL deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: Expiration date is one (1) year from date of opening.

Références pour anticorps anti-RFP (Biotin) (ABIN1607901)

Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." dans: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Abstract: RFP Produits

Sujet: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

ID gène: 55976617

UniProt: Q9U6Y8