SOD3 anticorps (AA 227-236)

N° du produit ABIN1686654

Détail du produit anti-SOD3 anticorps

Antigène: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Épitope: AA 227-236

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD3 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: Detects extracellular SOD ~35 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Peptide Affinity Purified

Immunogène: Peptide corresponding to AA 227-236 of human EC SOD

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1686654 was sufficient for detection of ECSOD in 20 μg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur SOD3

Antigène: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Autre désignation: SOD3 (SOD3 Produits)

Synonymes: anticorps EC-SOD, anticorps ECSOD, anticorps SOD3, anticorps AI314465, anticorps LOC780439, anticorps fb05f10, anticorps si:dkey-117m1.6, anticorps sod3, anticorps wu:fb05f10, anticorps ECSODPT, anticorps CuZnSOD, anticorps SODA.4, anticorps superoxide dismutase 3, anticorps superoxide dismutase 3, extracellular, anticorps superoxide dismutase 3, extracellular b, anticorps superoxide dismutase 1, anticorps superoxide dismutase precursor ,putative, anticorps SOD3, anticorps Sod3, anticorps sod3b, anticorps sod3, anticorps Sod1, anticorps Smp_095980

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

ID gène: 6649

UniProt: P08294