ANAPC10 anticorps (AA 31-130) (Biotin)

N° du produit ABIN1700786

Détail du produit anti-ANAPC10 anticorps (Biotin)

Antigène: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Épitope: AA 31-130

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ANAPC10 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Rat

Homologie: Human,Mouse,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human APC10

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur ANAPC10

Antigène: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Autre désignation: APC10 (ANAPC10 Produits)

Synonymes: anticorps APC10, anticorps DOC1, anticorps 1500026N15Rik, anticorps A830003M23Rik, anticorps CG11419, anticorps Dmel\\CG11419, anticorps ANAPC10, anticorps SPBC1E8.06, anticorps anapc10, anticorps MGC85037, anticorps DDBDRAFT_0202332, anticorps DDBDRAFT_0235160, anticorps DDB_0202332, anticorps DDB_0235160, anticorps anaphase promoting complex subunit 10, anticorps Anaphase Promoting Complex subunit 10, anticorps anaphase promoting complex subunit DOC1, anticorps anaphase-promoting complex subunit Apc10, anticorps anaphase promoting complex subunit, anticorps predicted protein, anticorps anaphase promoting complex subunit 10 S homeolog, anticorps ANAPC10, anticorps Anapc10, anticorps APC10, anticorps DOC1, anticorps apc10, anticorps CAALFM_C107850CA, anticorps anapc10, anticorps LOC692856, anticorps anapc10.S, anticorps NAEGRDRAFT_66759

Sujet:

Synonyms: ANAPC 10, anapc10, Anaphase promoting complex 10, Anaphase promoting complex subunit 10, Anaphase-promoting complex subunit 10, Apc 10, APC10, APC10_HUMAN, Cyclosome subunit 10, DKFZP564L0562, Doc 1, Doc1, OTTHUMP00000220297, OTTHUMP00000220298, OTTHUMP00000220299, OTTHUMP00000220300, OTTHUMP00000220303.

Background: Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

ID gène: 10393

Pathways: M Phase