RUNX3 anticorps (AA 31-130)

N° du produit ABIN1714069

Détail du produit anti-RUNX3 anticorps

Antigène: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Épitope: AA 31-130

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RUNX3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Runx3

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Independent Validation (IF)

Validation #029621 (Immunofluorescence)

by: CaresBio Laboratory

No.: #029621

Date: 08.03.2014

Numéro du lot: 131016

Application validée: Immunofluorescence

Contrôle positif: A431 cells

Contrôle négative: HEK293 cells

Conclusion: Strong signal was detected in the positive control, and no signal was detected in the negative control.

Validation Images

Figure 1: A431 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).

Figure 2: HEK293 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).

Figure 3: A431 cells stained with isotype control (green) and counterstained with DAPI (blue).

Figure 4: A431 cells stained with secondary antibody only (green) and counterstained with DAPI (blue).

Protocole

Anticorps primaire:

• Antigen: Runt-Related Transcription Factor 3 (RUNX3)
• Catalog number: ABIN1714069
• Lot number: 131016

Anticorps secondaire:

• Antibody: Donkey Anti-Rabbit secondary antibody IgG (H+L) AlexaFluor 488

Full Protocol:

• Antibody staining:
• A431 and Hek-293 cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
• Fixed cells were rinsed three times in PBS for 5 min each at RT.
• Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
• Cells were incubated with primary antibody diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.
• Isotype Control Staining:
  
• All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.
• Secondary Antibody Only Control Staining:
  
• All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with donkey anti rabbit Alexa488 conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.

Notes:

• No discrepancies reported.
• Runx3 antibody is recommended for IF on A431 cells according to this protocol.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur RUNX3

Antigène: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Autre désignation: Runx3 (RUNX3 Produits)

Synonymes: anticorps runxb, anticorps RUNX3, anticorps CBFA3, anticorps frrunx3, anticorps runx3, anticorps aml2, anticorps cbfa3, anticorps pebp2ac, anticorps AML2, anticorps Cbfa3, anticorps Pebp2a3, anticorps PEBP2aC, anticorps runt-related transcription factor 3, anticorps runt related transcription factor 3, anticorps runt related transcription factor 3 L homeolog, anticorps runx3, anticorps RUNX3, anticorps runx3.L, anticorps Runx3

Sujet:

Synonyms: RUNX 3, RUNX-3, Runt-related transcription factor 3, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, OncogeneAML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.

Background: RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.

ID gène: 864