ZBTB7A anticorps (AA 321-420)
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- Antigène Voir toutes ZBTB7A Anticorps
- ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))
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Épitope
- AA 321-420
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ZBTB7A est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Réactivité croisée
- Humain
- Homologie
- Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic peptide derived from human Pokemon
- Isotype
- IgG
- Top Product
- Discover our top product ZBTB7A Anticorps primaire
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- Indications d'application
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))
- Autre désignation
- Pokemon/ZBTB7 (ZBTB7A Produits)
- Synonymes
- anticorps ZBTB7A, anticorps FBI-1, anticorps FBI1, anticorps LRF, anticorps ZBTB7, anticorps ZNF857A, anticorps pokemon, anticorps 9030619K07Rik, anticorps 9130006G12Rik, anticorps AI452336, anticorps Lrf, anticorps Pokemon, anticorps Zbtb7, anticorps OCZF, anticorps zinc finger and BTB domain containing 7A, anticorps zinc finger and BTB domain containing 7C, anticorps zinc finger and BTB domain containing 7a, anticorps ZBTB7A, anticorps ZBTB7C, anticorps Zbtb7a
- Sujet
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Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.
Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
- ID gène
- 51341
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