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PCNA anticorps (AA 53-196)

Name: PCNA anticorps (AA 53-196)
Brand: antibodies-online
SKU: ABIN1724759
Price: 640.59 EUR
Availability: InStock

PCNA Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Souris Monoclonal 7H4F8 unconjugated

Western blot analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate. (PCNA anticorps (AA 53-196))

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA mouse mAb with DAB staining. (PCNA anticorps (AA 53-196))

IHC

Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple). (PCNA anticorps (AA 53-196))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PCNA anticorps (AA 53-196))

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS7 (7), and MCF-7 (8) cell lysate. (PCNA anticorps (AA 53-196))

Western blot analysis using PCNA mAb against human PCNA recombinant protein. (PCNA anticorps (AA 53-196))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA mouse mAb with DAB staining. (PCNA anticorps (AA 53-196))

IHC

7 images

(2 références)

N° du produit ABIN1724759

Fiche de données

Antigène Voir toutes PCNA Anticorps

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
Épitope
16

16

16

15

12

11

8

6

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 53-196
Reactivité
211

153

152

54

28

27

21

19

19

18

17

15

11

10

6

6

5

5

4

4

4

3

3

2

2

2

2

2

1

1

1

1

1
Humain
Hôte
130

94

3
Souris
Anticorps secondaires appropriés
Clonalité
129

97

1
Monoclonal
Conjugué
110

17

13

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp PCNA est non-conjugé
Application
189

68

63

59

52

48

46

40

30

15

15

12

5

4

3

2

2

2

2

2

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Fonction

PCNA Antibody

Purification

Ascitic fluid

Immunogène

Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Clone

7H4F8

Isotype

IgG1

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Indications d'application

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Takawa, Cho, Hayami, Toyokawa, Kogure, Yamane, Iwai, Maejima, Ueda, Masuda, Dohmae, Field, Tsunoda, Kobayashi, Akasu, Sugiyama, Ohnuma, Atomi, Ponder, Nakamura, Hamamoto: "Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression." dans: Cancer research, Vol. 72, Issue 13, pp. 3217-27, (2012) (PubMed).

Zhao, Ho, Lo, Espejo, Bedford, Hung, Wang: "Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer." dans: PLoS ONE, Vol. 7, Issue 1, pp. e29416, (2012) (PubMed).
Antigène

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation

PCNA (PCNA Produits)

Sujet

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Poids moléculaire

28.7kDa

ID gène

5111

HGNC

5111

UniProt

P12004

Pathways

Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy