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SQSTM1 anticorps (AA 232-356)

Name: SQSTM1 anticorps (AA 232-356)
Brand: antibodies-online
SKU: ABIN1724836
Price: 542.24 EUR
Availability: InStock

SQSTM1 Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Souris Monoclonal 5H7E2 unconjugated

Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate. (SQSTM1 anticorps (AA 232-356))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (SQSTM1 anticorps (AA 232-356))

ELISA

Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red). (SQSTM1 anticorps (AA 232-356))

FACS

Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein. (SQSTM1 anticorps (AA 232-356))

Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate. (SQSTM1 anticorps (AA 232-356))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SQSTM1 mouse mAb with DAB staining. (SQSTM1 anticorps (AA 232-356))

IHC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining. (SQSTM1 anticorps (AA 232-356))

IHC

7 images

(1 reference)

N° du produit ABIN1724836

Fiche de données

Antigène Voir toutes SQSTM1 Anticorps

SQSTM1 (Sequestosome 1 (SQSTM1))
Épitope
17

15

11

9

8

7

7

6

5

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 232-356
Reactivité
139

58

54

18

4

4

4

4

3

2

2

2

1

1

1

1
Humain
Hôte
116

21

1

1
Souris
Anticorps secondaires appropriés
Clonalité
115

24
Monoclonal
Conjugué
76

9

8

5

5

5

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp SQSTM1 est non-conjugé
Application
97

51

40

35

22

19

17

16

14

13

8

5

3

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Fonction

SQSTM1 Antibody

Purification

Purified antibody

Immunogène

Purified recombinant fragment of human SQSTM1 (AA: 232-356) expressed in E. Coli.

Clone

5H7E2

Isotype

IgG1

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Indications d'application

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Inoue, Suzuki, Mitsuishi, Miki, Suzuki, Sugawara, Watanabe, Sakurada, Endo, Uruno, Sasano, Nakagawa, Satoh, Tanaka, Kubo, Motohashi, Yamamoto: "Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma." dans: Cancer science, Vol. 103, Issue 4, pp. 760-6, (2012) (PubMed).
Antigène

SQSTM1 (Sequestosome 1 (SQSTM1))

Autre désignation

SQSTM1 (SQSTM1 Produits)

Sujet

Description: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Aliases: p60, p62, A170, OSIL, PDB3, ZIP3, p62B

Poids moléculaire

47.7kDa

ID gène

8878

HGNC

8878

UniProt

Q13501

Pathways

Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Autophagy