WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 100 up to 1 : 500 IHC: not tested yet
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Imbery, Bhattacharya, Khuder, Weiss, Goswamee, Iqbal, Giovannucci: "cAMP dependent recruitment of acidic organelles for Ca2+ signaling in the salivary gland." dans: American journal of physiology. Cell physiology, pp. ajpcell.00010.2016, (2016) (PubMed).
Matte, Casgrain, Séguin, Moradin, Hong, Descoteaux: "Leishmania major Promastigotes Evade LC3-Associated Phagocytosis through the Action of GP63." dans: PLoS pathogens, Vol. 12, Issue 6, pp. e1005690, (2016) (PubMed).
Demais, Barthélémy, Perraut, Ungerer, Keime, Reibel, Pfrieger: "Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 30, pp. 8012-25, (2016) (PubMed).
Golebiewska, Harper, Williams, Savage, Goggs, Fischer von Mollard, Poole: "Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability." dans: The Journal of biological chemistry, Vol. 290, Issue 3, pp. 1536-45, (2015) (PubMed).
Antigène
VAMP8
(Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8))