RAB3A anticorps (AA 154-227)

N° du produit ABIN1742210

Détail du produit anti-RAB3A anticorps

Antigène: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Épitope: AA 154-227

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAB3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for all four Rab 3 isoforms a, b, c, d.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Recombinant human Rab 3c (aa 154-227).

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC: 1 : 200 up to 1 : 500

Commentaires:

This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Références pour anticorps anti-RAB3A (ABIN1742210)

Romanov, Alpár, Zhang, Zeisel, Calas, Landry, Fuszard, Shirran, Schnell, Dobolyi, Oláh, Spence, Mulder, Martens, Palkovits, Uhlen, Sitte, Botting, Wagner, Linnarsson, Hökfelt, Harkany: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." dans: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, (2015) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." dans: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

de Wit, Walter, Milosevic, Gulyás-Kovács, Riedel, Sørensen, Verhage: "Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes." dans: Cell, Vol. 138, Issue 5, pp. 935-46, (2009) (PubMed).

Détails sur RAB3A

Antigène: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Autre désignation: Rab 3 (RAB3A Produits)

Synonymes: anticorps RAB3, anticorps rab3a, anticorps zgc:92276, anticorps RAB3A, anticorps rab-3a, anticorps si:ch211-255d18.4, anticorps zgc:112516, anticorps RAB3A, member RAS oncogene family, anticorps RAB3A, member RAS oncogene family, a, anticorps RAB3A, member RAS oncogene family L homeolog, anticorps RAB3A, member RAS oncogene family, b, anticorps RAB3A, anticorps Rab3a, anticorps rab3aa, anticorps rab3a.L, anticorps rab3a, anticorps rab3ab

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport