STX1B
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1 : 1000 (AP staining) ICC: 1 : 100 up to 1 : 1000 IHC: 1 : 1000 IHC-P: 1 : 500 ELISA: not tested yet
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS
Conseil sur la manipulation
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Stock
4 °C/-20 °C
Stockage commentaire
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Arsenault, Ferrari, Niranjan, Cuijpers, Gu, Vallis, OBrien, Davletov: "Stapling of the botulinum type A protease to growth factors and neuropeptides allows selective targeting of neuroendocrine cells." dans: Journal of neurochemistry, Vol. 126, Issue 2, pp. 223-33, (2013) (PubMed).
Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
Kabayama, Takeuchi, Taniguchi, Tokushige, Kozaki, Mizutani, Nakamura, Mikoshiba: "Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 20, pp. 7357-64, (2011) (PubMed).
Tsai, Garcia-Gil, van Haeften, Gadella: "How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane." dans: PLoS ONE, Vol. 5, Issue 6, pp. e11204, (2010) (PubMed).
Sander, Frank, Bolat, Blank, Galli, Bigalke, Bischoff, Lorentz: "Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells." dans: European journal of immunology, Vol. 38, Issue 3, pp. 855-63, (2008) (PubMed).
Kubota, Kobayashi, Mori, Higashide, McLaren, Inana: "Changes in retinal synaptic proteins in the transgenic model expressing a mutant HRG4 (UNC119)." dans: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 308-13, (2002) (PubMed).
Low, Marmorstein, Miura, Li, Kudo, Marmorstein, Weimbs: "Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype." dans: Journal of cell science, Vol. 115, Issue Pt 23, pp. 4545-53, (2002) (PubMed).