SNAP25 anticorps (AA 192-206)

N° du produit ABIN1742235

Détail du produit anti-SNAP25 anticorps

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Épitope: AA 192-206

Reactivité: Humain, Rat, Souris, Poulet, Poisson zèbre (Danio rerio), Hamster, Poisson rouge

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SNAP25 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Specific for SNAP 25.

Purification: antiserum

Immunogène: Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 10000 (AP staining)
ICC: 1 : 100

Commentaires:

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-SNAP25 (ABIN1742235)

Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." dans: eLife, Vol. 5, (2016) (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." dans: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." dans: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." dans: PLoS ONE, Vol. 7, Issue 5, pp. e36315, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." dans: PLoS ONE, Vol. 4, Issue 4, pp. e5310, (2009) (PubMed).

Johansson, Ericsson, Janson, Beraki, Stanić, Mandic, Wikström, Hökfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." dans: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, (2008) (PubMed).

Détails sur SNAP25

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Autre désignation: SNAP 25 (SNAP25 Produits)

Synonymes: anticorps RIC-4, anticorps RIC4, anticorps SEC9, anticorps SNAP, anticorps SNAP-25, anticorps bA416N4.2, anticorps dJ1068F16.2, anticorps SNAP-25B, anticorps SNAP-25a, anticorps ric-4, anticorps ric4, anticorps sec9, anticorps snap-25, anticorps Bdr, anticorps sp, anticorps fj55h11, anticorps snap25, anticorps wu:fj55h11, anticorps Snap, anticorps snap25.2, anticorps wu:fj33h05, anticorps synaptosome associated protein 25, anticorps synaptosome associated protein 25kDa S homeolog, anticorps synaptosomal-associated protein, 25kDa, anticorps synaptosomal-associated protein 25, anticorps synaptosome associated protein 25kDa, anticorps synaptosome associated protein 25kDa L homeolog, anticorps zgc:101731, anticorps synaptosomal-associated protein, 25b, anticorps SNAP25, anticorps Snap25, anticorps snap25.S, anticorps snap25, anticorps BACOIKO003_07, anticorps Tsp_10811, anticorps snap25.L, anticorps zgc:101731, anticorps snap25b

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport