NAPG
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
FITC
Indications d'application
WB: 1 : 500 up to 1 : 2000 (AP staining) ICC: not tested yet IHC: not tested yet
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).
Burgalossi, Jung, Meyer, Jockusch, Jahn, Taschenberger, OConnor, Nishiki, Takahashi, Brose, Rhee: "SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming." dans: Neuron, Vol. 68, Issue 3, pp. 473-87, (2010) (PubMed).