NMDA 1 Receptor anticorps (AA 35-53)
-
- Antigène Voir toutes NMDA 1 Receptor (NMDA R1) Anticorps
- NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))
-
Épitope
- AA 35-53
-
Reactivité
- Humain, Rat, Souris
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp NMDA 1 Receptor est non-conjugé
-
Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Specificité
- Specific for GluN 1.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Synthetic peptide (aa 35-53 of rat GluN 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
-
-
- Indications d'application
-
WB: 1 : 1000 (AP staining)
ICC: not recommended
IHC: not recommended - Commentaires
-
IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. IHC: Antibody 2, ABIN1742247, is recommended for IHC. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
-
-
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
: "Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey." dans: Behavioral and brain functions : BBF, Vol. 7, pp. 1, (2011) (PubMed).
: "
-
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
-
- Antigène
- NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))
- Autre désignation
- NMDA-Receptor 1 (NMDA R1 Produits)
- Synonymes
- anticorps CG2902, anticorps DMNMDAR1, anticorps DNMDAR-I, anticorps DNmdar, anticorps DmelNmdar1, anticorps Dmel\\CG2902, anticorps DrNR1, anticorps NMDA, anticorps NMDA R1, anticorps NMDAR1, anticorps NMdar1, anticorps NR1, anticorps NRI, anticorps Nmdar, anticorps dNR1, anticorps dnmdar-I, anticorps nmr, anticorps NMDA receptor 1, anticorps Nmdar1
-