SV2B anticorps (AA 2-17)

N° du produit ABIN1742258

Détail du produit anti-SV2B anticorps

Antigène: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Épitope: AA 2-17

Reactivité: Humain, Souris, Rat, Hamster, Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SV2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Specific for SV2 B.

Purification: antiserum

Immunogène: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
ICC: 1 : 200

Commentaires:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-SV2B (ABIN1742258)

Fukusumi, Wakamatsu, Takashima, Hasegawa, Miyauchi, Tomita, Kawachi: "SV2B is essential for the integrity of the glomerular filtration barrier." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 5, pp. 534-45, (2015) (PubMed).

Crèvecoeur, Kaminski, Rogister, Foerch, Vandenplas, Neveux, Mazzuferi, Kroonen, Poulet, Martin, Sadzot, Rikir, Klitgaard, Moonen, Deprez: "Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis." dans: Neuropathology and applied neurobiology, Vol. 40, Issue 2, pp. 191-204, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." dans: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Mahrhold, Strotmeier, Garcia-Rodriguez, Lou, Marks, Rummel, Binz: "Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E." dans: The Biochemical journal, Vol. 453, Issue 1, pp. 37-47, (2013) (PubMed).

Crèvecœur, Foerch, Doupagne, Thielen, Vandenplas, Moonen, Deprez, Rogister: "Expression of SV2 isoforms during rodent brain development." dans: BMC neuroscience, Vol. 14, pp. 87, (2013) (PubMed).

Couesnon, Molgó, Connan, Popoff: "Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine." dans: PLoS pathogens, Vol. 8, Issue 3, pp. e1002583, (2012) (PubMed).

Lazarevic, Schöne, Heine, Gundelfinger, Fejtova: "Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 28, pp. 10189-200, (2011) (PubMed).

Boido, Farisello, Cesca, Ferrea, Valtorta, Benfenati, Baldelli: "Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam." dans: Neuroscience, Vol. 171, Issue 1, pp. 268-83, (2010) (PubMed).

Yeh, Dong, Yao, Tepp, Lin, Johnson, Chapman: "SV2 mediates entry of tetanus neurotoxin into central neurons." dans: PLoS pathogens, Vol. 6, Issue 11, pp. e1001207, (2010) (PubMed).

Morgans, Kensel-Hammes, Hurley, Burton, Idzerda, McKnight, Bajjalieh: "Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina." dans: PLoS ONE, Vol. 4, Issue 4, pp. e5230, (2009) (PubMed).

Couesnon, Pereira, Popoff: "Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers." dans: Cellular microbiology, Vol. 10, Issue 2, pp. 375-87, (2008) (PubMed).

Verderio, Grumelli, Raiteri, Coco, Paluzzi, Caccin, Rossetto, Bonanno, Montecucco, Matteoli: "Traffic of botulinum toxins A and E in excitatory and inhibitory neurons." dans: Traffic (Copenhagen, Denmark), Vol. 8, Issue 2, pp. 142-53, (2007) (PubMed).

Miyauchi, Saito, Karasawa, Harita, Suzuki, Koike, Han, Shimizu, Kawachi: "Synaptic vesicle protein 2B is expressed in podocyte, and its expression is altered in proteinuric glomeruli." dans: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 10, pp. 2748-59, (2006) (PubMed).

Schivell, Mochida, Kensel-Hammes, Custer, Bajjalieh: "SV2A and SV2C contain a unique synaptotagmin-binding site." dans: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." dans: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Redecker, Kreutz, Bockmann, Gundelfinger, Boeckers: "Brain synaptic junctional proteins at the acrosome of rat testicular germ cells." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 6, pp. 809-19, (2003) (PubMed).

Janz, Südhof: "SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family." dans: Neuroscience, Vol. 94, Issue 4, pp. 1279-90, (2000) (PubMed).

Détails sur SV2B

Antigène: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Autre désignation: SV2 B (SV2B Produits)

Synonymes: anticorps SV2B, anticorps A830038F04Rik, anticorps AI850768, anticorps mKIAA0735, anticorps HsT19680, anticorps synaptic vesicle glycoprotein 2B, anticorps synaptic vesicle glycoprotein 2 b, anticorps synaptic vesicle glycoprotein 2b, anticorps SV2B, anticorps CpipJ_CPIJ009611, anticorps Sv2b