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UNC13A/Munc13-1 anticorps (AA 3-317)

UNC13A Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio) WB, IP, ICC, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1742277
  • Antigène Voir toutes UNC13A/Munc13-1 (UNC13A) Anticorps
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    Épitope
    • 2
    • 2
    • 1
    AA 3-317
    Reactivité
    Humain, Souris, Rat, Poisson zèbre (Danio rerio)
    Hôte
    • 3
    • 3
    • 1
    • 1
    Lapin
    Clonalité
    • 7
    • 1
    Polyclonal
    Conjugué
    • 8
    Cet anticorp UNC13A/Munc13-1 est non-conjugé
    Application
    • 8
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Specificité
    Specific for munc 13-1.
    Purification
    antiserum
    Immunogène
    Recombinant rat munc 13-1 (aa 3-317).
  • Indications d'application
    WB: 1 : 1000 (AP staining)
    ICC: 1 : 500
    IHC: 1 : 200
    ELISA: not tested yet
    Commentaires

    IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
    Buffer
    PBS
    Conseil sur la manipulation
    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

    Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." dans: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

    Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

  • Antigène
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    Autre désignation
    Munc 13-1 (UNC13A Produits)
    Synonymes
    anticorps Munc13-1, anticorps Unc13h1, anticorps 2410078G03Rik, anticorps unc-13 homolog A, anticorps unc-13 homolog A (C. elegans), anticorps Unc13a, anticorps UNC13A
    Pathways
    Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
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