UNC13A/Munc13-1 anticorps (AA 3-317)
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- Antigène Voir toutes UNC13A/Munc13-1 (UNC13A) Anticorps
- UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
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Épitope
- AA 3-317
- Reactivité
- Humain, Souris, Rat, Poisson zèbre (Danio rerio)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp UNC13A/Munc13-1 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- Specificité
- Specific for munc 13-1.
- Purification
- antiserum
- Immunogène
- Recombinant rat munc 13-1 (aa 3-317).
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- Indications d'application
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WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200
ELISA: not tested yet - Commentaires
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IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
- Buffer
- PBS
- Conseil sur la manipulation
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Stock
- 4 °C/-20 °C
- Stockage commentaire
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).
: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." dans: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).
: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).
: "
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Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).
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- Antigène
- UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
- Autre désignation
- Munc 13-1 (UNC13A Produits)
- Synonymes
- anticorps Munc13-1, anticorps Unc13h1, anticorps 2410078G03Rik, anticorps unc-13 homolog A, anticorps unc-13 homolog A (C. elegans), anticorps Unc13a, anticorps UNC13A
- Pathways
- Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
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