Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Specificité
Specific for APP.
Purification
antiserum
Immunogène
Synthetic peptide GYENPTYKFFEQMQN corresponding to the C-terminus of APP coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
APP
Reactivité: Souris
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1 : 500 up to 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500 IHC: not recommended IHC-P: not recommended
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS
Conseil sur la manipulation
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Stock
4 °C/-20 °C
Stockage commentaire
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Kumar, Wirths, Theil, Gerth, Bayer, Walter: "Early intraneuronal accumulation and increased aggregation of phosphorylated Abeta in a mouse model of Alzheimer's disease." dans: Acta neuropathologica, Vol. 125, Issue 5, pp. 699-709, (2013) (PubMed).