Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 596-705) anticorps

N° du produit ABIN1742305

Détail du produit

Antigène: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Épitope: AA 596-705

Reactivité: Souris, Rat, Humain, Poulet, Grenouille, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for RIM 1

Réactivité croisée (Details): weak cross reactivity to RIM 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Recombinant protein (aa 596-705) of rat RIM 1 containing part of PDZ domain.

Information d'application

Indications d'application: WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Référence

Tang, Chen, Li, Metzbower, MacGillavry, Blanpied: "A trans-synaptic nanocolumn aligns neurotransmitter release to receptors." dans: Nature, Vol. 536, Issue 7615, pp. 210-4, (2016) (PubMed).

Grabner, Gandini, Rehak, Le, Zamponi, Schmitz: "RIM1/2-Mediated Facilitation of Cav1.4 Channel Opening Is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 38, pp. 13133-47, (2015) (PubMed).

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Ramírez-Franco, Bartolomé-Martín, Alonso, Torres, Sánchez-Prieto: "Cannabinoid type 1 receptors transiently silence glutamatergic nerve terminals of cultured cerebellar granule cells." dans: PLoS ONE, Vol. 9, Issue 2, pp. e88594, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." dans: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Bello, Zanetti, Mayorga, Michaut: "RIM, Munc13, and Rab3A interplay in acrosomal exocytosis." dans: Experimental cell research, Vol. 318, Issue 5, pp. 478-88, (2012) (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." dans: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandstätter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." dans: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).

Jüttner, Moré, Das, Babich, Meier, Henning, Erdmann, Mu Ller, Otto, Grantyn, Rathjen: "Impaired synapse function during postnatal development in the absence of CALEB, an EGF-like protein processed by neuronal activity." dans: Neuron, Vol. 46, Issue 2, pp. 233-45, (2005) (PubMed).

Détail du antigène

Antigène: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Autre désignation: RIM 1 (RIMS1 Produits)

Synonymes: anticorps RIMS1, anticorps MGC121671, anticorps C030033M19Rik, anticorps RIM1, anticorps RIM1a, anticorps RIM1alpha, anticorps Rab3ip1, anticorps Rim, anticorps Serg1, anticorps mKIAA0340, anticorps CORD7, anticorps RAB3IP2, anticorps RIM, anticorps Rim1, anticorps regulating synaptic membrane exocytosis 1, anticorps RIMS1, anticorps rims1, anticorps Rims1

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity