RIMS2 anticorps (AA 461-987)
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- Antigène Voir toutes RIMS2 Anticorps
- RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))
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Épitope
- AA 461-987
- Reactivité
- Rat, Souris, Poulet, Hamster, Poisson zèbre (Danio rerio)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RIMS2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificité
- RIM 2 including splice variants
- Réactivité croisée (Details)
- cross reactivity to RIM 1.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Recombinant protein (aa 461-987) of rat RIM 2 containing part of PDZ domain.
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- Indications d'application
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WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 1000 - Commentaires
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This antibody works with paraformaldehyde and methanol fixation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." dans: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, (2014) (PubMed).
: "Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina." dans: Molecular vision, Vol. 20, pp. 1422-33, (2014) (PubMed).
: "Inter-channel scaffolding of presynaptic CaV2.2 via the C terminal PDZ ligand domain." dans: Biology open, Vol. 2, Issue 5, pp. 492-8, (2013) (PubMed).
: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).
: "Modulation of Cav1.3 Ca2+ channel gating by Rab3 interacting molecule." dans: Molecular and cellular neurosciences, Vol. 44, Issue 3, pp. 246-59, (2010) (PubMed).
: "Molecular anatomy of the hair cell's ribbon synapse." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).
: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." dans: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).
: "Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells." dans: Diabetes, Vol. 56, Issue 10, pp. 2579-88, (2007) (PubMed).
: "A proteomic screen for presynaptic terminal N-type calcium channel (CaV2.2) binding partners." dans: Journal of biochemistry and molecular biology, Vol. 40, Issue 3, pp. 302-14, (2007) (PubMed).
: "ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 6, pp. 659-72, (2006) (PubMed).
: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." dans: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, (2006) (PubMed).
: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." dans: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, (2005) (PubMed).
: "ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic ..." dans: Molecular biology of the cell, Vol. 16, Issue 7, pp. 3289-300, (2005) (PubMed).
: "
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Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." dans: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, (2014) (PubMed).
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- Antigène
- RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))
- Autre désignation
- RIM 2 (RIMS2 Produits)
- Pathways
- Hormone Transport, Synaptic Vesicle Exocytosis
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