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Bassoon anticorps (C-Term)

BSN Reactivité: Rat, Souris WB, ELISA, ICC Hôte: Souris Monoclonal 219E1 unconjugated
N° du produit ABIN1742310
  • Antigène Voir toutes Bassoon (BSN) Anticorps
    Bassoon (BSN)
    Épitope
    • 13
    • 2
    • 1
    • 1
    C-Term
    Reactivité
    • 25
    • 13
    • 13
    • 1
    • 1
    Rat, Souris
    Hôte
    • 25
    • 2
    Souris
    Clonalité
    • 25
    • 2
    Monoclonal
    Conjugué
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Bassoon est non-conjugé
    Application
    • 19
    • 18
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    Specificité
    Specific for bassoon.
    Purification
    hybridoma supernatant
    Immunogène
    Recombinant protein containing the 330 C-terminal aa of rat bassoon.
    Clone
    219E1
    Isotype
    IgG2b
    Top Product
    Discover our top product BSN Anticorps primaire
  • Indications d'application
    WB: 1 : 1000 (AP staining)
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 500
    IHC: not tested yet
    Commentaires

    ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS
    Conseil sur la manipulation
    When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
    Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.
    Stock
    -20 °C
    Stockage commentaire
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." dans: eLife, Vol. 5, (2016) (PubMed).

    Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." dans: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Schröder, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." dans: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).

    Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).

    Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

    Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).

  • Antigène
    Bassoon (BSN)
    Autre désignation
    Bassoon (BSN Produits)
    Synonymes
    anticorps Znf231, anticorps ZNF231, anticorps bassoon, anticorps bassoon (presynaptic cytomatrix protein), anticorps bassoon presynaptic cytomatrix protein, anticorps Bsn, anticorps BSN
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