SNAP91
Reactivité: Rat
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
AbBy Fluor® 555
Indications d'application
WB: 1 : 1000 up to 1 : 5000 (AP staining) IP: not tested yet ICC: 1 : 500 IHC: 1 : 1000
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." dans: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
Geis, Weishaupt, Hallermann, Grünewald, Wessig, Wultsch, Reif, Byts, Beck, Jablonka, Boettger, Üçeyler, Fouquet, Gerlach, Meinck, Sirén, Sigrist, Toyka, Heckmann, Sommer: "Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition." dans: Brain : a journal of neurology, Vol. 133, Issue 11, pp. 3166-80, (2010) (PubMed).
Zander, Münster-Wandowski, Brunk, Pahner, Gómez-Lira, Heinemann, Gutiérrez, Laube, Ahnert-Hilger: "Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 22, pp. 7634-45, (2010) (PubMed).
Antigène
SNAP91
(Synaptosomal-Associated Protein 91 (SNAP91))