Homer 1/2/3 (N-Term) anticorps
-
- Antigène
- Homer 1/2/3
- Épitope
- N-Term
- Reactivité
- Humain, Souris, Rat, Poisson zèbre (Danio rerio)
- Hôte
- Lapin
- Clonalité
- Polyclonal
- Application
- Immunocytochemistry (ICC), Western Blotting (WB)
- Specificité
- The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Purified recombinant N-terminal parts of Homer 1, 2 and 3.
-
- Indications d'application
-
WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
ELISA: not tested yet - Commentaires
-
ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
-
-
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." dans: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." dans: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).
: "
-
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." dans: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
-
- Antigène
- Homer 1/2/3
- Sujet
- Synonyms: VesL 1, Syn47
-