SYNPO
Reactivité: Rat, Souris
WB, FACS
Hôte: Lapin
Polyclonal
AbBy Fluor® 488
Indications d'application
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 600 IHC: 1 : 250 up to 1 : 500 IHC-P: 1 : 250 up to 1 : 500
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS
Conseil sur la manipulation
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Stock
4 °C/-20 °C
Stockage commentaire
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Sugar, Wassenhove-McCarthy, Orr, Green, van Kuppevelt, McCarthy: "N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions." dans: American journal of physiology. Renal physiology, Vol. 310, Issue 10, pp. F1123-35, (2016) (PubMed).
Fester, Zhou, Ossig, Labitzke, Bläute, Bader, Vollmer, Jarry, Rune: "Synaptopodin is regulated by aromatase activity." dans: Journal of neurochemistry, Vol. 140, Issue 1, pp. 126-139, (2016) (PubMed).
Collier, OMalley, Rademacher, Stancati, Sisson, Sortwell, Paumier, Gebremedhin, Steece-Collier: "Interrogating the aged striatum: robust survival of grafted dopamine neurons in aging rats produces inferior behavioral recovery and evidence of impaired integration." dans: Neurobiology of disease, Vol. 77, pp. 191-203, (2015) (PubMed).
Dieni, Nestel, Sibbe, Frotscher, Hellwig: "Distinct synaptic and neurochemical changes to the granule cell-CA3 projection in Bassoon mutant mice." dans: Frontiers in synaptic neuroscience, Vol. 7, pp. 18, (2015) (PubMed).
Spada, Rojas, Pérez-Yagüe, Mulens, Cannata-Ortiz, Bragado, Barber: "NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery." dans: Journal of leukocyte biology, Vol. 97, Issue 3, pp. 583-98, (2015) (PubMed).
Sun, Qu, Zhang, Caruana, Bertram, Li: "Glomerular endothelial cell injury and damage precedes that of podocytes in adriamycin-induced nephropathy." dans: PLoS ONE, Vol. 8, Issue 1, pp. e55027, (2013) (PubMed).
Cohen, Louneva, Han, Hodes, Wilson, Bennett, Lucki, Arnold: "Chronic corticosterone exposure alters postsynaptic protein levels of PSD-95, NR1, and synaptopodin in the mouse brain." dans: Synapse (New York, N.Y.), Vol. 65, Issue 8, pp. 763-70, (2011) (PubMed).
Chen, Wassenhove-McCarthy, Yamaguchi, Holzman, van Kuppevelt, Orr, Funk, Woods, McCarthy: "Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices." dans: Kidney international, Vol. 78, Issue 11, pp. 1088-99, (2010) (PubMed).
Tarabra, Giunti, Barutta, Salvidio, Burt, Deferrari, Gambino, Vergola, Pinach, Perin, Camussi, Gruden: "Effect of the monocyte chemoattractant protein-1/CC chemokine receptor 2 system on nephrin expression in streptozotocin-treated mice and human cultured podocytes." dans: Diabetes, Vol. 58, Issue 9, pp. 2109-18, (2009) (PubMed).
Vlachos, Korkotian, Schonfeld, Copanaki, Deller, Segal: "Synaptopodin regulates plasticity of dendritic spines in hippocampal neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 4, pp. 1017-33, (2009) (PubMed).