VAMP5
Reactivité: Humain
IHC, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1 : 1000 up to 1 : 5000 (AP staining) IP: not tested yet ICC: 1 : 1000 IHC-P: 1 : 200
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Ikezawa, Tajika, Ueno, Murakami, Inoue, Yorifuji: "Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung." dans: Developmental dynamics : an official publication of the American Association of Anatomists, (2017) (PubMed).
Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." dans: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, (2015) (PubMed).
Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." dans: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, (2013) (PubMed).
Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, (2009) (PubMed).
Antigène
VAMP5
(Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))