Reactivité: Souris, Rat, Boeuf (Vache), Humain, Singe
IHC, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Carrott, Bowl, Aguilar, Johnson, Chessum, West, Morse, Dorning, Smart, Hardisty-Hughes, Ball, Parker, Barnard, MacLaren, Wells, Marcotti, Brown: "Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 1, pp. 222-34, (2016) (PubMed).
Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." dans: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
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Regus-Leidig, Fuchs, Löhner, Leist, Leal-Ortiz, Chiodo, Hauswirth, Garner, Brandstätter: "In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses." dans: Frontiers in cellular neuroscience, Vol. 8, pp. 259, (2014) (PubMed).
Tekin, Chioza, Matsumoto, Diaz-Horta, Cross, Duman, Kokotas, Moore-Barton, Sakoori, Ota, Odaka, Foster, Cengiz, Tokgoz-Yilmaz, Tekeli, Grigoriadou, Petersen, Sreekantan-Nair, Gurtz, Xia, Pandya et al.: "SLITRK6 mutations cause myopia and deafness in humans and mice. ..." dans: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2094-102, (2013) (PubMed).
Fuchs, Scholz, Sendelbeck, Atorf, Schlegel, Enz, Brandstätter: "Rod photoreceptor ribbon synapses in DBA/2J mice show progressive age-related structural changes." dans: PLoS ONE, Vol. 7, Issue 9, pp. e44645, (2012) (PubMed).
Hübler, Rankovic, Richter, Lazarevic, Altrock, Fischer, Gundelfinger, Fejtova: "Differential spatial expression and subcellular localization of CtBP family members in rodent brain." dans: PLoS ONE, Vol. 7, Issue 6, pp. e39710, (2012) (PubMed).
Regus-Leidig, tom Dieck, Brandstätter: "Absence of functional active zone protein Bassoon affects assembly and transport of ribbon precursors during early steps of photoreceptor synaptogenesis." dans: European journal of cell biology, Vol. 89, Issue 6, pp. 468-75, (2010) (PubMed).