TNR
Reactivité: Humain
Hôte: Lapin
Polyclonal
FITC
Indications d'application
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Stock
-20 °C
Stockage commentaire
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Arranz, Perkins, Irie, Lewis, Hrabe, Xiao, Itano, Kimata, Hrabetova, Yamaguchi: "Hyaluronan deficiency due to Has3 knock-out causes altered neuronal activity and seizures via reduction in brain extracellular space." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 18, pp. 6164-76, (2014) (PubMed).
Apostolova, Irintchev, Schachner: "Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 30, pp. 7849-59, (2006) (PubMed).
Schuster, Krug, Stalder, Hackel, Gerardy-Schahn, Schachner et al.: "Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix ..." dans: Journal of neurobiology, Vol. 49, Issue 2, pp. 142-58, (2001) (PubMed).
Weber, Bartsch, Rasband, Czaniera, Lang, Bluethmann, Margolis, Levinson, Shrager, Montag, Schachner: "Mice deficient for tenascin-R display alterations of the extracellular matrix and decreased axonal conduction velocities in the CNS." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 11, pp. 4245-62, (1999) (PubMed).
Antigène
Tenascin R (TNR)
(Tenascin R (Restrictin, Janusin) (TNR))