Abeta-pE3 anticorps
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- Antigène
- Abeta-pE3
- Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificité
- Specific for Abeta-pE3.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
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- Indications d'application
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WB: 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500
IHC-P: 1 : 200 - Commentaires
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WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." dans: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, (2014) (PubMed).
: "Glutaminyl cyclase-mediated toxicity of pyroglutamate-beta amyloid induces striatal neurodegeneration." dans: BMC neuroscience, Vol. 14, pp. 108, (2013) (PubMed).
: "Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β." dans: Nature, Vol. 485, Issue 7400, pp. 651-5, (2012) (PubMed).
: "Involvement of perineuronal and perisynaptic extracellular matrix in Alzheimer's disease neuropathology." dans: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 4, pp. 547-61, (2012) (PubMed).
: "Glutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-A? deposits in hippocampus via distinct cellular mechanisms." dans: Acta neuropathologica, Vol. 121, Issue 6, pp. 705-19, (2011) (PubMed).
: "Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated Aβ is induced by pyroglutamate-Aβ formation." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 36, pp. 12790-801, (2011) (PubMed).
: "
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Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." dans: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, (2014) (PubMed).
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- Antigène
- Abeta-pE3
- Sujet
- Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)
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