CHGB
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
HRP
Indications d'application
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500 IHC-P: 1 : 200
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Alvarez-Saavedra, De Repentigny, Yang, OMeara, Yan, Hashem, Racacho, Ioshikhes, Bulman, Parks, Kothary, Picketts: "Voluntary Running Triggers VGF-Mediated Oligodendrogenesis to Prolong the Lifespan of Snf2h-Null Ataxic Mice." dans: Cell reports, Vol. 17, Issue 3, pp. 862-875, (2016) (PubMed).
Farina, van de Bospoort, He, Persoon, van Weering, Broeke, Verhage, Toonen: "CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons." dans: eLife, Vol. 4, (2015) (PubMed).
Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." dans: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).