ZYX anticorps (AA 352-357)

N° du produit ABIN1742558

Détail du produit anti-ZYX anticorps

Antigène: ZYX (Zyxin (ZYX))

Épitope: AA 352-357

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZYX est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Specific for zyxin.

Purification: purified IgG. Azide was added before lyophilization.

Immunogène: Recombinant human zyxin. Epitope TLKEVE (aa 352-357 of human zyxin)

Clone: 164D4

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 : 100 up to 1 : 2000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-ZYX (ABIN1742558)

Missirlis, Haraszti, Scheele, Wiegand, Diaz, Neubauer, Rechenmacher, Kessler, Spatz: "Substrate engagement of integrins α5β1 and αvβ3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin." dans: Scientific reports, Vol. 6, pp. 23258, (2016) (PubMed).

Gadhari, Charnley, Marelli, Brugger, Chiquet: "Cell shape-dependent early responses of fibroblasts to cyclic strain." dans: Biochimica et biophysica acta, Vol. 1833, Issue 12, pp. 3415-25, (2013) (PubMed).

Delaroche, Cantrelle, Subra, Van Heijenoort, Guittet, Jiao, Blanchoin, Chassaing, Lavielle, Auclair, Sagan: "Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts." dans: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7712-21, (2010) (PubMed).

Low, Mukhina, Srinivas, Ng, Murata-Hori: "Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis." dans: Experimental cell research, Vol. 316, Issue 12, pp. 1925-34, (2010) (PubMed).

Mori, Nakagami, Koibuchi, Miura, Takami, Koriyama, Hayashi, Sabe, Mochizuki, Morishita, Kaneda: "Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis." dans: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3115-24, (2009) (PubMed).

Vollenbröker, George, Wolfgart, Saleem, Pavenstädt, Weide: "mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes." dans: American journal of physiology. Renal physiology, Vol. 296, Issue 2, pp. F418-26, (2009) (PubMed).

Vervenne, Crombez, Delvaux, Janssens, Van de Ven, Petit: "Targeted disruption of the mouse Lipoma Preferred Partner gene." dans: Biochemical and biophysical research communications, Vol. 379, Issue 2, pp. 368-73, (2009) (PubMed).

Guo, Wang: "Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals." dans: Molecular biology of the cell, Vol. 18, Issue 11, pp. 4519-27, (2007) (PubMed).

Coutts, MacKenzie, Griffith, Black: "TES is a novel focal adhesion protein with a role in cell spreading." dans: Journal of cell science, Vol. 116, Issue Pt 5, pp. 897-906, (2003) (PubMed).

Garvalov, Higgins, Sutherland, Zettl, Scaplehorn, Köcher, Piddini, Griffiths, Way: "The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions." dans: The Journal of cell biology, Vol. 161, Issue 1, pp. 33-9, (2003) (PubMed).

Rottner, Krause, Gimona, Small, Wehland: "Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions." dans: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3103-13, (2001) (PubMed).

Détails sur ZYX

Antigène: ZYX (Zyxin (ZYX))

Autre désignation: Zyxin (ZYX Produits)

Synonymes: anticorps BcDNA:LD06023, anticorps CG32018, anticorps DLim-1, anticorps DmLIM-1, anticorps Dmel\\CG32018, anticorps Lim102EF, anticorps ZYX102, anticorps Zyx102, anticorps Zyx102EF, anticorps lim, anticorps zyx102, anticorps zyxin, anticorps zgc:171680, anticorps ZYX, anticorps zyx, anticorps MGC145270, anticorps ESP-2, anticorps HED-2, anticorps 9530098H06Rik, anticorps R75157, anticorps Zyxin, anticorps zyxin, anticorps zyxin S homeolog, anticorps zyx-1, anticorps Zyx, anticorps zyx, anticorps ZYX, anticorps EDI_201420, anticorps zyx.S