Profilin anticorps (AA 42-47)

N° du produit ABIN1742560

Détail du produit anti-Profilin anticorps

Antigène: Profilin (PFN)

Épitope: AA 42-47

Reactivité: Birch

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Profilin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Specific for birch profilin.

Purification: purified IgG

Immunogène: Recombinant birch profilin. Epitope PQFKPQ (aa 42-47)

Clone: 4A6

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1 : 500 up to 1 : 10000
IHC: not tested yet

Commentaires:

This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-Profilin (ABIN1742560)

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." dans: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Détails sur Profilin

Antigène: Profilin (PFN)

Autre désignation: Profilin (PFN Produits)

Synonymes: anticorps LOC406154, anticorps GB13380, anticorps profilin, anticorps PFN, anticorps PRF