ELISA. Immunohistochemistry on paraffin sections: 1: 100. Western Blot: 1: 1,500, detects a band of approximately 28 kDa under reducing conditions. Astrong band of 88 kDa representing glycosylated FeLV gag gene product may also bepresent. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0mg/mL
Buffer
PBS, pH 7.2 containing 0.05 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
FeLV Gag
Classe de substances
Viral Protein
Sujet
The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.Synonyms: Feline Leukemia Virus