Nanog anticorps (Biotin)

N° du produit ABIN181657

Détail du produit anti-Nanog anticorps (Biotin)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nanog est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes Human Nanog. Other species not tested.

Purification: Affinity Chromatography

Immunogène: Highly pure (> 98%) E.coli recombinant Human Nanog

Information d'application

Indications d'application: ELISA: Direct: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Sandwich: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNanog as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNanog. Western blot: To detect hNanog this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG (NANOG Produits)

Synonymes: anticorps 2410002E02Rik, anticorps ENK, anticorps ecat4, anticorps NANOG, anticorps hacp, anticorps wu:fd19e04, anticorps wu:fd20a07, anticorps zgc:193933, anticorps Nanog homeobox, anticorps nanog homeobox, anticorps Nanog, anticorps NANOG, anticorps nanog

Sujet: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

ID gène: 100293888

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance