DEFB1
Reactivité: Humain, Rat
IF (cc), IF (p)
Hôte: Lapin
Polyclonal
AbBy Fluor® 350
Indications d'application
ELISA: Direct: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1. Sandwich: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1(AP01151PU) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human BD-1. Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1