CCL25
Reactivité: Souris
Hôte: Lapin
Polyclonal
FITC
Indications d'application
ELISA: Direct: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Sandwich: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTECK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTECK. Western blot: To detect hTECK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Conseil sur la manipulation
This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
Stock
4 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!