Notch1 anticorps
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- Antigène Voir toutes Notch1 (NOTCH1) Anticorps
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Notch1 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody is specific for Notch1, a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow.
- Purification
- Affinity Chromatography on Protein G
- Immunogène
- Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- MN1A
- Isotype
- IgG1
- Top Product
- Discover our top product NOTCH1 Anticorps primaire
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- Indications d'application
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Western Blot. Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections (1/50-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservataive.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Autre désignation
- NOTCH1 (NOTCH1 Produits)
- Synonymes
- anticorps notch1, anticorps NOTCH1, anticorps hn1, anticorps tan1, anticorps notch, anticorps xotch, anticorps xnotch, anticorps notch-1, anticorps xnotch1, anticorps TAN1, anticorps hN1, anticorps 9930111A19Rik, anticorps Mis6, anticorps N1, anticorps Tan1, anticorps lin-12, anticorps NOTCH, anticorps Xotch, anticorps notch1-a, anticorps notch 1, anticorps fibropellin-1, anticorps Notch homolog 1, translocation-associated (Drosophila), anticorps Notch homolog 1, translocation-associated, anticorps notch 1 S homeolog, anticorps notch1, anticorps LOC100631526, anticorps NOTCH1, anticorps Notch1, anticorps notch1.S
- Sujet
- The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.Synonyms: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
- ID gène
- 4851
- NCBI Accession
- NP_060087
- UniProt
- P46531
- Pathways
- Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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