Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.
Attributs du produit
Molecular Weight: Predicted Band Size 60 kDa, Observed Band Size 60 kDa
Purification
Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Synthesized phosphopeptide derived from human Smad2 around pSer467 (C-S-S-M-SP)
SMAD2
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500-1:1,000
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage., Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody at -20 °C. It will remain stable for one year.
Song, Thalacker, Nilsen-Hamilton: "Synergistic and multidimensional regulation of plasminogen activator inhibitor type 1 expression by transforming growth factor type ? and epidermal growth factor." dans: The Journal of biological chemistry, Vol. 287, Issue 15, pp. 12520-8, (2012) (PubMed).
Antigène
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))