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NCF1 anticorps (C-Term)

Verified NCF1 Reactivité: Humain, Souris, Porc WB, ELISA, IHC, IF, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN184583
  • Antigène Voir toutes NCF1 Anticorps
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    Épitope
    • 28
    • 16
    • 12
    • 11
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 128
    • 48
    • 44
    • 16
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    Humain, Souris, Porc
    Hôte
    • 140
    • 2
    • 1
    Chèvre
    Clonalité
    • 140
    • 2
    Polyclonal
    Conjugué
    • 73
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp NCF1 est non-conjugé
    Application
    • 104
    • 65
    • 60
    • 26
    • 24
    • 17
    • 14
    • 13
    • 8
    • 8
    • 4
    • 4
    • 3
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Fonction
    NCF1 / p47phox
    Séquence
    SESTKRKLAS AV
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Porc, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

    Peptide ELISA: antibody detection limit dilution 1:20000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    Autre désignation
    NCF1 (NCF1 Produits)
    Synonymes
    anticorps NCF1A, anticorps NOXO2, anticorps SH3PXD1A, anticorps p47phox, anticorps Ncf-1, anticorps p47, anticorps neutrophil cytosolic factor 1, anticorps NCF1, anticorps Ncf1
    Sujet
    NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
    ID gène
    653361, 17969, 114553
    NCBI Accession
    NP_000256
    Pathways
    Signalisation PI3K-Akt
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