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FOXP3 anticorps (C-Term)

Name: FOXP3 anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN184677
Price: 779.48 EUR
Availability: InStock

Verified FOXP3 Reactivité: Humain WB, FACS, ELISA, IHC, IF, ICC Hôte: Chèvre Polyclonal unconjugated

ABIN184677 (1µg/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1µg/ml) negative control Pancreas (C) lysate (35µg protein in RIPA buffer). (FOXP3 anticorps (C-Term))

ABIN184677 (1µg/ml) staining of paraffin embedded Human Tonsil. (FOXP3 anticorps (C-Term))

IHC

ABIN184677 staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B). (FOXP3 anticorps (C-Term))

ICC

ABIN184677 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (FOXP3 anticorps (C-Term))

ABIN184677 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0. (FOXP3 anticorps (C-Term))

FACS

5 images

N° du produit ABIN184677

Fiche de données

Antigène Voir toutes FOXP3 Anticorps

FOXP3 (Forkhead Box P3 (FOXP3))
Épitope
22

16

15

9

8

6

6

6

6

6

6

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivité
163

123

51

26

6

3

2

2

1

1

1

1

1

1

1

1
Humain
Hôte
112

69

20

1
Chèvre
Anticorps secondaires appropriés
Clonalité
112

90
Polyclonal
Conjugué
89

19

14

13

11

7

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
Cet anticorp FOXP3 est non-conjugé
Application
128

91

70

38

37

27

27

18

16

15

9

8

8

7

4

3

2

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Fonction

FOXP3 / SCURFIN

Séquence

SQRPSRCSNP TPGP

Specificité

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Réactivité croisée

Humain

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité

Verified

Immunogène

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

Isotype

IgG

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Indications d'application

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat
Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Minimize freezing and thawing.

Stock

-20 °C

Stockage commentaire

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Antigène

FOXP3 (Forkhead Box P3 (FOXP3))

Autre désignation

FOXP3 (FOXP3 Produits)

Synonymes

anticorps foxp3, anticorps FOXP3, anticorps AIID, anticorps DIETER, anticorps IPEX, anticorps PIDX, anticorps XPID, anticorps JM2, anticorps scurfin, anticorps sf, anticorps RGD1562112, anticorps forkhead box P3, anticorps forkhead box P3a, anticorps forkhead box P3 L homeolog, anticorps FOXP3, anticorps foxp3a, anticorps foxp3, anticorps foxp3.L, anticorps Foxp3

Sujet

FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin

ID gène

50943

NCBI Accession

NP_054728, NP_001107849

Pathways

Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation