KIF5B anticorps (C-Term)

N° du produit ABIN184774

Détail du produit anti-KIF5B anticorps

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KIF5B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Fonction: Kinesin 1 / UKHC

Séquence: QPVAVRGGGG KQV

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-KIF5B (ABIN184774)

Connell, Allison, Rodger, Pearson, Zlamalova, Reid: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." dans: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." dans: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Yasuda, Clatterbuck-Soper, Jackrel, Shorter, Mili: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." dans: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." dans: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." dans: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

Détails sur KIF5B

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Autre désignation: KIF5B (KIF5B Produits)

Synonymes: anticorps KINH, anticorps KNS, anticorps KNS1, anticorps UKHC, anticorps Khc, anticorps AL022807, anticorps Khcs, anticorps Kns1, anticorps Ukhc, anticorps khc, anticorps KIF5A, anticorps kinesin family member 5B, anticorps kinesin heavy chain, anticorps kinesin-1, anticorps kinesin-1 heavy chain, anticorps kinesin family member 5B S homeolog, anticorps KIF5B, anticorps LOC411769, anticorps cgd6_2790, anticorps kin-1, anticorps Tb927.1.1350, anticorps LACBIDRAFT_305999, anticorps Khc, anticorps LOC100538629, anticorps Kif5b, anticorps kif5b.S

Sujet: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain

ID gène: 3799

NCBI Accession: NP_004512

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization