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UBE2C anticorps (C-Term)

Verified UBE2C Reactivité: Humain WB, ELISA, IF, IP, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN184805
  • Antigène Voir toutes UBE2C Anticorps
    UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
    Épitope
    • 15
    • 11
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 71
    • 16
    • 15
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 59
    • 12
    Chèvre
    Clonalité
    • 58
    • 13
    Polyclonal
    Conjugué
    • 36
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp UBE2C est non-conjugé
    Application
    • 57
    • 32
    • 17
    • 13
    • 13
    • 10
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)
    Fonction
    UBE2C / UBCH10
    Séquence
    QETYSKQVTS QEP
    Specificité
    This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
     Réactivité croisée
    Humain
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
    Isotype
    IgG
    Top Product
    Discover our top product UBE2C Anticorps primaire
  • Indications d'application

    Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
    Autre désignation
    UBE2C (UBE2C Produits)
    Synonymes
    anticorps LOC458287, anticorps UBCH10, anticorps dJ447F3.2, anticorps 1110015A16Rik, anticorps D2Ertd695e, anticorps im:7137135, anticorps zgc:123190, anticorps ubiquitin conjugating enzyme E2 C, anticorps ubiquitin-conjugating enzyme E2C, anticorps ubiquitin conjugating enzyme E2 C L homeolog, anticorps ubiquitin conjugating enzyme E2 C S homeolog, anticorps UBE2C, anticorps NAEGRDRAFT_82946, anticorps ube2c.L, anticorps Ube2c, anticorps ube2c.S, anticorps ube2c
    Sujet
    UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
    ID gène
    11065
    NCBI Accession
    NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670
    Pathways
    Ubiquitin Proteasome Pathway
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