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ARIH2 anticorps (C-Term)

Verified ARIH2 Reactivité: Humain, Souris WB, ELISA, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN184916
  • Antigène Voir toutes ARIH2 Anticorps
    ARIH2 (protein ariadne-2 homolog (ARIH2))
    Épitope
    • 9
    • 8
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 48
    • 20
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    Humain, Souris
    Hôte
    • 44
    • 4
    Chèvre
    Clonalité
    • 45
    • 2
    Polyclonal
    Conjugué
    • 27
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ARIH2 est non-conjugé
    Application
    • 35
    • 26
    • 23
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Fonction
    ARIH2 / TRIAD1
    Séquence
    EQRRRTLLKD FHDT
     Réactivité croisée
    Boeuf (Vache), Chien, Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.
    Isotype
    IgG
    Top Product
    Discover our top product ARIH2 Anticorps primaire
  • Indications d'application

    Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Commentaires

    Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." dans: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

  • Antigène
    ARIH2 (protein ariadne-2 homolog (ARIH2))
    Autre désignation
    ARIH2 (ARIH2 Produits)
    Synonymes
    anticorps ARI2, anticorps TRIAD1, anticorps AI843547, anticorps UIP48, anticorps wu:fa09c12, anticorps zgc:64093, anticorps ariadne RBR E3 ubiquitin protein ligase 2, anticorps ariadne RBR E3 ubiquitin protein ligase 2 L homeolog, anticorps ariadne homolog 2 (Drosophila), anticorps ARIH2, anticorps Arih2, anticorps arih2.L, anticorps arih2
    Sujet
    ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
    ID gène
    10425, 23807
    NCBI Accession
    NP_006312
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