PSMF1 anticorps (C-Term)
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- Antigène Voir toutes PSMF1 Anticorps
- PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))
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Épitope
- C-Term
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Reactivité
- Humain, Souris, Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PSMF1 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Fonction
- PI31 / PSMF1 (Isoform 1)
- Séquence
- DHLPPPGYDD MYL
- Specificité
- This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
- Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Classe de qualité
- Verified
- Immunogène
- C-DHLPPPGYDDMYL
- Isotype
- IgG
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- Indications d'application
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Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293, approx 30-32 kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35 kDa in lysates of cell line LNCaP (calculated MW of 29.8 kDa according to Human NP_006805.2 an
Peptide ELISA: antibody detection limit dilution 1:32000.
- Commentaires
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Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry:A customer reported weak staining of A431 cells at
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Minimize freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Antigène
- PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))
- Autre désignation
- PSMF1 (PSMF1 Produits)
- Synonymes
- anticorps PI31, anticorps AW048666, anticorps BC012260, anticorps zgc:92785, anticorps proteasome inhibitor subunit 1, anticorps proteasome (prosome, macropain) inhibitor subunit 1, anticorps PSMF1, anticorps Psmf1, anticorps psmf1
- Sujet
- PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1
- ID gène
- 9491, 228769
- NCBI Accession
- NP_006805
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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