Pleckstrin anticorps (C-Term)

N° du produit ABIN185127

Détail du produit anti-Pleckstrin anticorps

Antigène: Pleckstrin (PLEK)

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Pleckstrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: Pleckstrin

Séquence: EWIKAIQMAS RTGK

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: C-EWIKAIQMASRTGK

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40-45 kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1 kDa according to Human NP_002655.2). Recommended concentration: 0.0

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Pleckstrin

Antigène: Pleckstrin (PLEK)

Autre désignation: PLEK (PLEK Produits)

Synonymes: anticorps MGC69065, anticorps PLEK, anticorps P47, anticorps 2010300B13Rik, anticorps pleckstrin L homeolog, anticorps pleckstrin, anticorps plek.L, anticorps PLEK, anticorps Plek

Sujet: PLEK, pleckstrin, P47, p47, FLJ27168

ID gène: 5341

NCBI Accession: NP_002655

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process