KPNA3 anticorps (C-Term)

N° du produit ABIN185159

Détail du produit anti-KPNA3 anticorps

Antigène: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: KPNA3 / IPOA4

Séquence: DPTANLQTKE FNF

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-DPTANLQTKEFNF, from the C Terminus of the protein sequence according to NP_002258.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.

Western Blot: Approx. 60 kDa band observed in lysates of cell lines CaCo-2, HEK293 and MCF7 (calculated MW of 57.8 kDa according to NP_002258.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-KPNA3 (ABIN185159)

Jovičić, Mertens, Boeynaems, Bogaert, Chai, Yamada, Paul, Sun, Herdy, Bieri, Kramer, Gage, Van Den Bosch, Robberecht, Gitler: "Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS." dans: Nature neuroscience, Vol. 18, Issue 9, pp. 1226-9, (2015) (PubMed).

Ohara, Hata, Yasuhara, Mehmood, Yoneda, Nakagawa, Yamashita: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." dans: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." dans: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Détails sur KPNA3

Antigène: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Autre désignation: KPNA3 (KPNA3 Produits)

Synonymes: anticorps IPOA4, anticorps si:dz79f24.2, anticorps importin, anticorps SRP1, anticorps SRP1gamma, anticorps SRP4, anticorps hSRP1, anticorps ipoa4, anticorps srp1, anticorps srp1gamma, anticorps srp4, anticorps karyopherin (importin) alpha 3, anticorps karyopherin alpha 3 (importin alpha 4), anticorps karyopherin subunit alpha 3, anticorps karyopherin alpha 3 (importin alpha 4) L homeolog, anticorps Kpna3, anticorps kpna3, anticorps KPNA3, anticorps kpna3.L

Sujet: KPNA3, IPOA4, karyopherin alpha 3 (importin alpha 4), RP11-432M24.3, SRP1gamma, SRP4, hSRP1, importin alpha 4, importin alpha 4, importin-alpha-Q2, karyopherin alpha 3, SRP1, importin alpha-3

ID gène: 3839, 16648

NCBI Accession: NP_002258

Pathways: Protein targeting to Nucleus