IFT74 anticorps (C-Term)

N° du produit ABIN185213

Détail du produit anti-IFT74 anticorps

Antigène: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFT74 est non-conjugé

Application: ELISA, Western Blotting (WB)

Fonction: CMG1 / CCDC2 / IFT74

Séquence: KTIVDALHST SGN

Réactivité croisée: Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2 kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-IFT74 (ABIN185213)

Boldt, Mans, Won, van Reeuwijk, Vogt, Kinkl, Letteboer, Hicks, Hurd, Naggert, Texier, den Hollander, Koenekoop, Bennett, Cremers, Gloeckner, Nishina, Roepman, Ueffing: "Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice." dans: The Journal of clinical investigation, Vol. 121, Issue 6, pp. 2169-80, (2011) (PubMed).

Momeni, Schymick, Jain, Cookson, Cairns, Greggio, Greenway, Berger, Pickering-Brown, Chiò, Fung, Holtzman, Huey, Wassermann, Adamson, Hutton, Rogaeva, St George-Hyslop, Rothstein, Hardiman, Grafman et al.: "Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. ..." dans: BMC neurology, Vol. 6, pp. 44, (2007) (PubMed).

Détails sur IFT74

Antigène: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Autre désignation: IFT74 (IFT74 Produits)

Sujet: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

ID gène: 80173, 313365

NCBI Accession: NP_001092692, NP_001092693, NP_079379

Pathways: Signalisation Hedgehog, Chromatin Binding