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Androgen Receptor anticorps (N-Term)

Verified AR Reactivité: Humain WB, ELISA, IHC, IF, ChIP Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185266
  • Antigène Voir toutes Androgen Receptor (AR) Anticorps
    Androgen Receptor (AR)
    Épitope
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    N-Term
    Reactivité
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    Humain
    Hôte
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    Chèvre
    Clonalité
    • 248
    • 66
    Polyclonal
    Conjugué
    • 120
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    Cet anticorp Androgen Receptor est non-conjugé
    Application
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
    Fonction
    Androgen Receptor
    Séquence
    EVQLGLGRVY PRPPSC
    Specificité
    This antibody is expected to recognize isoform 1 (NP_000035.2) only.
    Homologie
    Human, Mouse, Rat, Dog, Pig, Cow
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
    Isotype
    IgG
    Top Product
    Discover our top product AR Anticorps primaire
  • Indications d'application

    DS WB Results: Approx 100 kDa band observed in Human Cerebellum lysates (calculated MW of 99.2 kDa according to NP_000035.2). Recommended concentration:0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

    DS IHC Results: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

    Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    Androgen Receptor (AR)
    Autre désignation
    Androgen Receptor (AR Produits)
    Synonymes
    anticorps AIS, anticorps DHTR, anticorps HUMARA, anticorps HYSP1, anticorps KD, anticorps NR3C4, anticorps SBMA, anticorps SMAX1, anticorps TFM, anticorps AW320017, anticorps Tfm, anticorps Andr, anticorps AR, anticorps androgen receptor, anticorps AR, anticorps Ar, anticorps ar
    Sujet
    AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
    ID gène
    367
    NCBI Accession
    NP_000035
    Pathways
    Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding
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